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Image Search Results
Journal: Molecular Medicine
Article Title: Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway
doi: 10.1186/s10020-024-00845-4
Figure Lengend Snippet: Wogonin inhibited TLR4 to ameliorate renal injury via upregulation of SOCS3 in db/db mice. db/db mice were injected with adenovirus sh-SOCS3 or sh-SOCS3 + sh-TLR4, followed by treatment by vehicle or wogonin. ( A ) Blood glucose levels were measured using an automated chemistry analyzer. ( B ) The pathological features and glucose in the renal tissues were evaluated by hematoxylin, eosin (HE), and periodic acid-Schiff base (PAS). Scale bar = 50 μm. ( C - D ) The urinary protein, serum creatinine values (Scr), and blood urea nitrogen (BUN) were evaluated by an automated chemistry analyzer. ( E - F ) 24-hour albumin and urinary albumin-to-creatinine ratio (ACR) were detected by ELISA assay in HG mice without or with wogonin, wogonin + sh-SOCS3, wogonin + sh-SOCS3 + sh-TLR4 ( E ) ELISA evaluated the levels of cytokines. ( F - G ) The mRNA and protein levels of SOCS3 and TLR4 were detected by RT-qPCR and western blot, respectively. ( H ) The protein levels of collagen I, CTGF, FN, and TGF-β1 were detected by western blot assay. ( I ) The protein levels of ACE, Ang II, and AT1R were detected by western blot assay. ( J ) The protein levels of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, AIM2, ASC, caspase-1, IL-18 and IL-1β were detected by western blot assay. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the corresponding control. Data are representative of three independent experiments
Article Snippet: Subsequently, hematoxylin and eosin were used to analyze the
Techniques: Injection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Control
Journal: International Journal of Biological Sciences
Article Title: Ferroptosis inhibitor alleviates sorafenib-induced cardiotoxicity by attenuating KLF11-mediated FSP1-dependent ferroptosis
doi: 10.7150/ijbs.86479
Figure Lengend Snippet: Sorafenib treatment induces ferroptosis in H9c2 rat cardiomyocytes. (A) Live and dead assays. Green, Calcein AM, live cells. Red, PI, dead cells. Scale bar, 100 μm. (B) Measurement of LDH release in H9c2 cells. (C) Western blot analysis and quantification of FSP1, GPX4 and GAPDH (negative control). (D) mRNA expression level of FSP1 was detected by qPCR. (E) mRNA expression level of GPX4 was detected by qPCR. (F) Evaluation of mitochondrial morphology using Mito Tracker Green fluorescent probe. (magnification: upper panel scale bar = 10 μm, lower panel scale bar = 5 μm). (G) DCFH-DA staining was used to detect ROS production in different experimental groups. Scale bar, 50 μm. (H) Lipid peroxidation in H9c2 cells was determined by the level of MDA. (I) Transmission electron microscope pictures (magnification: upper panel scale bar = 5 μm, lower panel scale bar = 1 μm). * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Live cell staining was carried out using
Techniques: Western Blot, Negative Control, Expressing, Staining, Transmission Assay, Microscopy